FlowJo Vx

FlowJo's backgating analysis provides a tool to view the effect of every gate in the gating tree of a multi-generation gated population.
FlowJo generates graphs within which each level in the gating hierarchy is displayed, with the final gated population overlaid at each level.

This overlay is a backgating overlay: in other words, it shows the final gated population within the population of its ancestors.